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Bioprocess Engineering: Basic Concepts by Shuler and Kargi
Bioprocess engineering is a field of engineering that uses biological and chemical processes to design and create systems, devices, or products that interact with living cells or materials[^4^]. Bioprocess engineers work in various industries, such as biotechnology, biopharmaceutical, food, and alternative fuels, to research, improve, and manage the production of bioproducts[^5^].
One of the most popular and comprehensive textbooks on bioprocess engineering is Bioprocess Engineering: Basic Concepts by Michael L. Shuler and Fikret Kargi. This book introduces the essential concepts of bioprocessing, such as internal structure and functions of different types of microorganisms, major metabolic pathways, enzymes, microbial genetics, kinetics and stoichiometry of growth and product formation, and bioreactor design and operation. It also explores the engineering principles necessary for bioprocess synthesis and design, and illustrates the application of these principles to modern biotechnology for production of pharmaceuticals and biologics, solution of environmental problems, production of commodities, and medical applications[^1^].
The book is suitable for undergraduate and graduate students in chemical engineering, biological engineering, bioengineering, biotechnology, and biochemical engineering. It is also a valuable reference for professionals and researchers in the bioprocess industry. The book has been updated to include the latest developments and advances in the field, such as metabolic engineering, synthetic biology, systems biology, biofuels, biosensors, nanobiotechnology, and biopharmaceuticals[^2^].
If you are interested in learning more about bioprocess engineering and its applications, you can download the book from the following link: Bioprocess Engineering: Basic Concepts by Shuler and Kargi [^3^]. You can also find other related books and resources on Google Books or Pearson website.
In this article, we will review some of the main topics covered in the book Bioprocess Engineering: Basic Concepts by Shuler and Kargi. These topics include:
Microbial cell structure and function: This topic covers the basic features and functions of different types of microorganisms, such as bacteria, fungi, algae, and animal cells. It also explains the role of cell membranes, cell walls, cytoplasm, ribosomes, nucleoid, plasmids, mitochondria, chloroplasts, and other organelles in cellular activities.
Enzymes and enzyme kinetics: This topic covers the structure and function of enzymes, which are biological catalysts that speed up chemical reactions in living cells. It also covers the factors that affect enzyme activity, such as substrate concentration, temperature, pH, inhibitors, and activators. It also introduces the mathematical models and methods to describe and analyze enzyme kinetics, such as Michaelis-Menten equation, Lineweaver-Burk plot, and Eadie-Hofstee plot.
Major metabolic pathways: This topic covers the major metabolic pathways that occur in living cells, such as glycolysis, pentose phosphate pathway, tricarboxylic acid cycle (TCA cycle), electron transport chain (ETC), oxidative phosphorylation (OXPHOS), fermentation, photosynthesis, and respiration. It also explains how these pathways are regulated and integrated to produce energy and biomass for cellular growth and maintenance.
Microbial genetics and recombinant DNA technology: This topic covers the basic principles of microbial genetics, such as DNA structure and replication, RNA synthesis and processing, protein synthesis and folding, gene expression and regulation, mutation and repair, genetic recombination and transfer. It also covers the techniques and applications of recombinant DNA technology, such as restriction enzymes, plasmids, vectors, cloning, transformation, transfection, gene editing (CRISPR-Cas9), gene expression systems (E. coli, yeast, mammalian cells), and protein purification methods.
These topics provide a solid foundation for understanding the biological aspects of bioprocess engineering. In the next article, we will review some of the engineering aspects of bioprocess engineering. ec8f644aee